| 转谷氨酰胺酶2抑制H1亚型流感病毒在MDCK细胞中的增殖 |
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| 引用本文： | 郭寿清,廖月姣,仇真毓,刘耿,王家敏,杨迪,张家友,乔自林,马忠仁,李倬,刘振斌. 转谷氨酰胺酶2抑制H1亚型流感病毒在MDCK细胞中的增殖[J]. 生物工程学报, 2022, 38(3): 1124-1137 |
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| 作者姓名： | 郭寿清 廖月姣 仇真毓 刘耿 王家敏 杨迪 张家友 乔自林 马忠仁 李倬 刘振斌 |
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| 作者单位： | 西北民族大学 生物医学研究中心甘肃省动物细胞技术创新中心, 甘肃 兰州 730030;西北民族大学 生命科学与工程学院, 甘肃 兰州 730030;西北民族大学 生物医学研究中心甘肃省动物细胞技术创新中心, 甘肃 兰州 730030;西北民族大学 生物医学研究中心 生物工程与技术国家民委重点实验室, 甘肃 兰州 730030 |
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| 基金项目： | 甘肃省科技计划(20JR10RA121)；国家自然科学基金地区科学基金(32160164)；中央高校基本科研业务费专项资金(31920210033)；甘肃省重点研发计划-社会发展类(21JR1RA222) |
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| 摘 要： | 组织转谷酰胺酶(transglutaminase 2,TGM2)是一种普遍存在的多功能蛋白,与不同细胞的粘附和肿瘤形成有关.有证据表明,TGM2参与了宿主细胞与病毒间的相互作用,但是对于流感病毒在细胞内增殖的影响还未有报道.为了探究MDCK细胞中TGM2对H1N1亚型流感病毒增殖的影响,本研究构建了TGM2过表达和敲除...
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| 关 键 词： | TGM2 MDCK细胞 H1N1亚型流感病毒 |
| 收稿时间： | 2021-09-07 |
| 修稿时间： | 2021-11-30 |
Transglutaminase 2 inhibits the proliferation of H1 subtype influenza virus in MDCK cells |
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| GUO Shouqing,LIAO Yuejiao,QIU Zhenyu,LIU Geng,WANG Jiamin,YANG Di,ZHANG Jiayou,QIAO Zilin,MA Zhongren,LI Zhuo,LIU Zhenbin. Transglutaminase 2 inhibits the proliferation of H1 subtype influenza virus in MDCK cells[J]. Chinese journal of biotechnology, 2022, 38(3): 1124-1137 |
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| Authors: | GUO Shouqing LIAO Yuejiao QIU Zhenyu LIU Geng WANG Jiamin YANG Di ZHANG Jiayou QIAO Zilin MA Zhongren LI Zhuo LIU Zhenbin |
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| Affiliation: | Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu Univerity, Lanzhou 730030, Gansu, China;Life Science and Engineering College, Northwest Minzu University, Lanzhou 730030, Gansu, China;Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu Univerity, Lanzhou 730030, Gansu, China;Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, Gansu, China |
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| Abstract: | Transglutaminase 2(TGM2) is a ubiquitous multifunctional protein,which is related to the adhesion of different cells and tumor formation.Previous studies found that TGM2 is involved in the interaction between host cells and viruses,but the effect of TGM2 on the proliferation of influenza virus in cells has not been reported.To explore the effect of TGM2 during H1N1 subtype influenza virus infection,a stable MDCK cell line with TGM2 overexpression and a knockout cell line were constructed.The mRNA and protein expression levels of NP and NS1 as well as the virus titer were measured at 48 hours after pot-infection with H1N1 subtype influenza virus.The results showed that overexpression of TGM2 effectively inhibited the expression of NP and NS1 genes of H1N1 subtype influenza virus,while knockout of TGM2 up-regulated the expression of the NP and NS1 genes,and the expression of the NP at protein level was consistent with that at mRNA level.Virus proliferation curve showed that the titer of H1N1 subtype influenza virus decreased significantly upon TGM2 overexpression.On the contrary,the virus titer in TGM2 knockout cells reached the peak at 48 h,which further proved that TGM2 was involved in the inhibition of H1N1 subtype influenza virus proliferation in MDCK cells.By analyzing the expression of genes downstream of influenza virus response signaling pathway,we found that TGM2 may inhibit the proliferation of H1N1 subtype influenza virus by promoting the activation of JAK-STAT molecular pathway and inhibiting RIG-1 signaling pathway.The above findings are of great significance for revealing the mechanism underlying the interactions between host cells and virus and establishing a genetically engineering cell line for high-yield influenza vaccine production of influenza virus. |
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| Keywords: | TGM2 MDCK cells H1N1 subtype influenza virus |
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