| 高氧预适应对大鼠心肌缺血─再灌注时自由基的影响 |
| |
| 引用本文： | 英明中,李小鹰,王士雯.高氧预适应对大鼠心肌缺血─再灌注时自由基的影响[J].中国应用生理学杂志,1996(3). |
| |
| 作者姓名： | 英明中 李小鹰 王士雯 |
| |
| 作者单位： | 解放军总医院南楼监护室 |
| |
| 摘 要： | 为了解决高氧预适应（ＨｙｐｅｒｏｘｉｃｐｒｅｃｏｎｄｉｔｉｏｎｉｎｇＨＯＰ）对大鼠心肌缺血再灌注时自由基的影响，本实验将实验组大鼠放入高压氧舱内，每日吸８０－８５％氧气（１ａｔｍ）６ｈ，连续７ｄ。利用Ｌａｎｇｅｎｄｏｒｆ装置做成心肌缺血再灌注模型，采用电子自旋共振技术测定自由基含量。实验动物随机分为二组，第一组为对照组：缺血１０ｍｉｎ，再灌注６０ｍｉｎ。第二组为ＨＯＰ组：缺血１０ｍｉｎ，再灌注６０ｍｉｎ。实验观察冠脉回流液中自由基ＰＢＮ加合物含量。结果表明：在再灌注过程中，１、５、１０ｍｉｎ３个时间点，ＨＯＰ组ＰＢＮ加合物含量较对照组明显减少。提示：ＨＯＰ能减少缺血再灌注时自由基的产生。
|
| 关 键 词： | 高氧预适应 缺血─再灌注 损伤 自由基 电子自旋 共振 |
EFFECT OF HYPEROXIC PRECONDITIONING ON FREE RADICAL GENERATION DURING MYOCARDIAL ISCHEMIA REPERFUSION IN RATS |
| |
| Yin mingzhong,Li xiaoying,Wang shiwen.EFFECT OF HYPEROXIC PRECONDITIONING ON FREE RADICAL GENERATION DURING MYOCARDIAL ISCHEMIA REPERFUSION IN RATS[J].Chinese Journal of Applied Physiology,1996(3). |
| |
| Authors: | Yin mingzhong Li xiaoying Wang shiwen |
| |
| Abstract: | In order to observe the effect of hyeroxic preconditioning(HOP) on free radical(FR) generated by ischemia reperfusion, rats were exposed to 80-85% O 2(1 atm) six hours a day for 7 days. The control rats were exposed to room air. Both groups were killed and their hearts were isolated and under gone 10 min ischemia followed by 60 min reperfusion by Langendorff method. RP PBN adducts were measured by electron spin resonance(ESR) at baseline, 1, 5, and 10 min of reperfusion. The results showed that PBN adducts in HOP group were significantly less than that in control group. It is concluded that oxygen free radicals were reduced by HOP during myocardial ischemia reperfusion in rats. |
| |
| Keywords: | hyeroxic preconditioning(HOP) ischemia reperfusion injury free radical ESR |
| 本文献已被 CNKI 等数据库收录！ |
