Multiplex PCR-RFLP assay for detection of factor V Leiden and prothrombin G20210A |
| |
Authors: | Baris Ibrahim Koksal Vedat Etlik Ozdal |
| |
Affiliation: | Department of Molecular Biology and Genetics, Bogazici University, 34342, Istanbul, Turkey. ibrahim.baris@boun.edu.tr |
| |
Abstract: | Factor V Leiden and prothrombin G20210A are clinically relevant genetic risk factors for venous thrombosis. Molecular diagnostic testing for factor V Leiden and prothrombin G20210A is widespread, and laboratories use a variety of technical approaches. Here we introduce a multiplex polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) based on single (Mn/l) restriction endonuclease digestion. The assay was shown to simultaneously and accurately detect factor V Leiden and prothrombin G20210A mutations. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|