Evidence for a DNA ligase change related to early cleavage in axolotl egg |
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| Affiliation: | 1. Laboratoire de Biochimie du Développment, Institut de Recherches en Biologie Moléculaire, Université Paris VII, 2 place Jussieu, 75221 Paris Cedex 05 France;2. Laboratoire de Biologie du Développment, Université de Caen, Esplanade de la Paix, 14032 Caen Cedex France;1. The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL, USA;2. Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, FL, USA |
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| Abstract: | A definite change in the forms of DNA ligase appears when the axolotl egg enters cleavage. Sucrose gradient and phosphocellulose chromatography show that the a 6S form of DNA ligase exists before division, i.e. in unfertilised and fertilised egg, and a 8.2S form is present at the first division. N-ethylmaleimide sensitivity and heat stability are different for the two forms. The possible significance of this early change is discussed. |
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