Cell cycle dependent distribution of the proliferation-associated Ki-67 antigen in human embryonic lung cells |
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Authors: | Norbert Braun Thomas Papadopoulos Hans Konrad Müller-Hermelink |
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Affiliation: | 1. Institute of Pathology, Julius-Maximilians Universit?t, Josef-Schneider-Stra?e 2, D-8700, Würzburg, Federal Republic of Germany
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Abstract: | The cell cycle-dependent distribution of the proliferation-associated Ki-67 antigen has been evaluated immunocytochemically in L-132 human fetal lung cells. The cells were synchronized and cell cycle phases were determined: G1 = 6.7 h, S = 5.4 h, G2 = 8.5 h and mitosis = 1.3 h. The Ki-67 patterns were strictly correlated with the cell cycle phases. In late G1-phase, Ki-67 antigen was present only in the perinucleolar region. In the S-phase, Ki-67 staining was found homogeneously in the karyoplasm and in the perinucleolar region. G2-phase cells contained a finely granular Ki-67 staining in the karyoplasm with Ki-67-positive specks and perinucleolar staining. In early mitotic cells (pro- and metaphase) an intense perichromosomal Ki-67 staining was observed in addition to a homogeneously stained karyoplasm in prophase, and cytoplasm in metaphase. During ana- and telophase the Ki-67 antigen disappeared rapidly. In resting cells there was no Ki-67 staining. |
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Keywords: | Ki-67 Immunocytochemistry Cell cycle Cell proliferation Synchronization |
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