Occurrence of an oxalate oxidase in Sorghum leaves |
| |
Authors: | Chandra S Pundir Ravindra Nath |
| |
Institution: | Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India |
| |
Abstract: | An oxalate oxidase found in the 15 000 g supernatant of 10-day-old sorghum leaves exhibited a pH optimum of 5 and a temperature optimum of 45° and was unaffected by Na+. The enzyme activity remained linear up to 10 min and the apparent Km for oxalate was 2.4 × 10?5 M. The enzyme activity was strongly inhibited by sodium dithionite and α,α′-dipyridyl. Inhibition by the latter was specifically reversed by Fe2+. The activity of the dialysed enzyme was restored by the addition of Fe2+ and FAD. Inhibition of the enzyme by iodoacetate, p-chloromercuribenzoate and N-methylmaleimide revealed that SH groups at the active site are essential. |
| |
Keywords: | Gramineae sorghum leaf oxalate oxidation oxalate oxidase SH groups |
本文献已被 ScienceDirect 等数据库收录! |