The glycolipid fatty acids of Porphyridium purpureum cultured in the presence of detergents |
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Authors: | Harri Nyberg Kristiina Koskimies-Soininen |
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Institution: | Department of Botany, University of Helsinki, Unioninkatu 44, SF-00170 Helsinki 17, Finland |
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Abstract: | The glycolipid fatty acid composition of Porphyridium purpureum on a solid medium was studied in the presence of Triton X-100 (TX), sodium desocycholate (SDC), sodium dodecyl sulphate (SDS) and cetyl trimethylammonium bromide (CTAB). TLC and GC/MS were used in determining the relative fatty acid compositions of mono-and digalactosyl diglycerides (MGDG and DGDG) and in assessing the MGDG/DGDG ratio. The most common fatty acids were palmitic (16:0), stearic (18:0), linoleic (18:2 ω6), arachidonic (20:4 ω6) and eicosapentaenoic (20:5 ω3) acids, the long-chain polyunsaturated acids being more abundant in DGDG; α-linolenic acid (18:3 ω3) was absent. TX and SDC in particular caused an increase in the saturation grade of both MGDG and DGDG fatty acids at very low concentrations (5–15 ppm). With a detergent concentration of 20 ppm a reversion of this tendency was sometimes found, and the fatty acid composition approached the controls again. The effects of SDS and CTAB were not as prominent. All the detergents studied increased the normal MGDG/DGDG ratio (0.3) to a maximum of ~ 1. The effect of increasing detergent concentration is not linear. The results suggest that in some cases very low detergent concentrations can be more effective than higher ones, a fact which may be important in algae growing in polluted waters. |
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Keywords: | Rhodophyceae cell culture glycolipids fatty acids detergents Abbreviations used throughout are: MGDG monogalactosyldiglycerides DGDG digalactosyldiglycerides TX Triton X-100 SDC sodium desoxycholate SDS sodium dodecyl sulphate CTAB cetyl trimethylammonium bromide |
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