Tetrapyrrole biosynthesis in Anacystis nidulans; incorporation of [1-13C]-, [2-13C]-, [1,2-13C]- and [2-13C, 2-2H3]acetate |
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Authors: | Norman G. Lewis John A. Walter Jeffrey L.C. Wright |
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Affiliation: | Atlantic Research Laboratory, National Research Council, 1411 Oxford Street, Halifax, N.S., B3H 3Z1 Canada |
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Abstract: | Labelling experiments with [2-13C]- and [1,2-13C]acetate showed that both photopigments of Anacystis nidulans, chlorophyll a and phycocyanobilin, share a common biosynthetic pathway from glutamate. The fate of deuterium during these biosynthetic events was studied using [2-13C, 2-2H3]acetate as a precursor and determining the labelling pattern by 13C NMR spectroscopy with simultaneous [1H, 2H]-broadband decoupling. The loss of 2H (ca 20%) from the precursor occurred at an early stage during the tricarboxylic acid cycle. After formation of glutamate there was no further loss of 2H in the assembly of the cyclic tetrapyrrole intermediates or during decarboxylation and modification of the side-chains. Thus the labelling data support a divergence in the pathway to cyclic and linear tetrapyrroles after protoporphyrin IX. |
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Keywords: | Cyanophyta blue-green algae biosynthesis stable isotopes tetrapyrroles phycocyanobilin tricarboxylic acid cycle. |
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