Difference in extractability of estradiol- and tamoxifen-receptor complex in the nuclei from MCF-7 cells with nonidet P-40 |
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Authors: | Mitsunori Ikeda Yoshikazu Omukai Keiichi Hosokawa Tsuneaki Senoo |
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Institution: | 1. Department of Biochemistry, Kawasaki Medical School, 577 Matsushima, Kurashiki 701-01 (Japan);2. Department of Endocrine Surgery. Kawasaki Medical School, 577 Matsushima, Kurashiki 701-01 (Japan) |
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Abstract: | The extraction of 3H]estradiol- and 3H]tamoxifen-receptor complex in the nuclei from MCF-7 cells with the nonionic detergent Nonidet P-40 has been studied. We found that there is a striking difference in the extractability of estradiol- and tamoxifen-receptor complex from nuclei with 0.5% Nonidet P-40. The nuclear bound estradiol-receptor complex is scarcely extractable with Nonidet P-40. In contrast, almost all of the nuclear bound tamoxifen-receptor complex is extractable. The nuclear 3H]tamoxifen-receptor complex extracted in the presence of Nonidet P-40 sediments in two peaks at 7 S and 5 S. The latter sedimentation rate is the same with that of the nuclear 3H]tamoxifen-receptor complex extracted with 0.4 M KC1. The nuclear 3H]estradiol-receptor complex extracted with 0.4 M KC1 sediments at 4 S.The results suggest that interaction of tamoxifen-receptor complex with chromatin is different from that of estradiol-receptor complex. |
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