Isolation and characterization of a lectin from the seeds of Erythrina Edulis |
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Authors: | Pérez Gerardo |
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Affiliation: | Biochemistry Section, Chemistry Department, Universidad Nacional Bogotá, Colombia |
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Abstract: | A galactose-specific lectin was isolated from the seeds of Erythrina edulis. The protein was purified by affinity chromatography of the globulin fraction on an allyl-galactoside polyacrylamide gel. The hemagglutination properties, amino acid composition, A280, MW of the protein and of its subunits, carbohydrate content, electrophoretic pattern and isoelectric point were determined. Comparison of its properties with those of other Erythrina lectins shows that the protein is a distinct member of this group of lectins. |
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Keywords: | Leguminosae isolation characterization lectin. |
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