Growth on indium-tin oxide-coated glass enhances 32P-phosphate uptake and protein labelling of adherent cells |
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Authors: | Tomai E Klein S Firth K Raptis L |
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Institution: | Queen's University, Dept of Microbiology and Immunology, Kingston, Ont, Canada. |
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Abstract: | A method to improve the efficiency of labelling of adherent cells with radioactive 32p is described. Cells are grown on a glass surface which is coated with indium-tin oxide, a commercially available, transparent material which permits excellent cell adhesion and growth. The results show that a 2 to 3-fold increase in 32p uptake by the cells can be achieved by growing cells on this material, compared to conventional tissue culture plastic. |
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