Probing the MVAI methyltransferase region that interacts with DNA: affinity labeling with the dialdehyde-containing DNA duplexes |
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Authors: | Gritsenko O M Mikhailov S N Efimtseva E V Van Aerschot A Herdewijn P Gromova E S |
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Institution: | Department of Chemistry, Moscow State University, Russia. |
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Abstract: | Affinity labeling of methyltransferase MvaI by DNA duplexes containing oxidized 2'-O-beta-D-ribofuranosylcytidine or 1-beta-D-galactopyranosyl)thymine residues was performed. Partial chemical hydrolysis of the covalently bound methylase in the conjugates with the dialdehyde-containing DNA allowed us to determine the amino acid region in the C terminus of methylase MvaI that interacts with DNA. |
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