Antisense phosphorothioate oligonucleotides targeted to the human chemokine receptor CXCR4 |
| |
Authors: | Kusunoki A Miyano-Kurosaki N Kimura T Takai K Yamamoto N Gushima H Takaku H |
| |
Affiliation: | Department of Industrial Chemistry, Chiba Institute of Technology, Narashino, Japan. |
| |
Abstract: | The CXC chemokine receptor CXCR4 is used as a major co-receptor for fusion and entry by syncytia-inducing T-tropic (X4) isolates of HIV-1. In the present study, we report the effects of an antisense oligodeoxyribonucleotide on the inhibition of CXCR4 gene expression in X4 HIV-1 infected HeLa-CD4 cells, to find more efficacious therapeutic possibilities for Human Immunodeficiency Virus type 1 (HIV-1) infection. Antisense phosphorothioate oligodeoxyribonucleotides (anti-S-ODNs) corresponding to the sequence of bases 69 to 88 of the human CXCR4 mRNA gene were synthesized. When the naked anti-S-ODN was incubated with HeLa-CD4 cells, the surface levels of this chemokine receptor were reduced up to 50%, indicating sequence-specific inhibition. We also examined the concomitant use of a basic peptide transfection reagent, nucleosomal histone proteins (RNP), for delivery of anti-S-ODNs. The anti-S-ODN encapsulated with RNP had higher inhibitory effects on p24 products than the naked anti-S-ODN. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|