5-HT和GnRH及其受体在长牡蛎卵巢的生理作用机制——双染和免疫细胞化学定位

用免疫细胞化学与双染技术对5-HT和GnRH及其受体在长牡蛎卵巢中的分布的研究结果提示，不同发育时期卵巢中卵母细胞均存在5-HT和GnRH受体，受体阳性物质沿胞膜分布，显深棕色，核显免疫阴性反应；5-HT和GnRH及其共存于不同时期卵母细胞胞质和胞膜上。以上结果表明长牡蛎的卵母细胞不仅能产生5-HT和GnRH，而且能表达5-HT和GnRH的受体，5-HT和GnRH可能以自分泌的方式对卵母细胞起调节作用。

Mechanism of 5-HT and GnRH Physiological Action in the Ovary of Crassostrea gigas:Orientation of Double Staining and Immunocytochemistry

Distribution of 5-HT,GnRH and their receptors within ovaries of Crassostrea gigas was studied using the immunocytochemistry and double staining technique.The results revealed that 1)5-HT and GnRH receptors existed in the oocytes of different development stages of the ovary;2)the reactive substances of 5-HT and GnRH receptors were distributed along the membrane of oocytes and showed dark brown,but nucleus showed immunonegative reaction;3)5-HT,GnRH and their receptors co-existed in the cytoplasm and membrane of oocytes of different development stages.The above results indicate that the oocytes of Crassostrea gigas can not only produce 5-HT and GnRH,but also express their receptors.5-HT and GnRH may exert regulation action on the oocytes by autosecretion.