Determination of the anti-tumor agent, 10-chloro-5-(2-dimethylaminoethyl)-7H-indolo[2,3-C] quinolin-6(5H)-one in blood or plasma by high-performance liquid chromatography

A sensitive and specific high-performance liquid chromatographic assay was developed for the determination of 10-chloro-5-(2-dimethylaminoethyl)-7H-indolo2,3-C] quinolin-6(5H)-one I] in blood or plasma with an overall recovery of 100.3 ± 9.1% and a limit of quantitation of 1.0 ng per ml of blood or plasma. The assay was used to determine blood concentrations of the drug in the rat following oral administration by intubation of a 1.17-mg dose of I] - HCl.