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INSECTICIDE RESISTANCE IN THE GROUND SPIDER,Pardosa sumatrana (THORELL, 1890; ARANEAE: LYCOSIDAE)
Authors:Hafiz Muhammad Tahir  Farva Khizar  Sajida Naseem  Rabia Yaqoob  Khizar Samiullah
Affiliation:1. Department of Zoology, GC University, Lahore, Pakistan;2. Department of Zoology, University of Sargodha, Sargodha, Pakistan;3. Department of Zoology, GC University, Faisalabad, Pakistan
Abstract:Elevated levels of insecticides detoxifying enzymes, such as esterases, glutathione S‐transferases (GSTs), and cytochrome P‐450 monooxygenases, act in the resistance mechanisms in insects. In the present study, levels of these enzymes in the insecticide‐resistant ground spider Pardosa sumatrana (Thorell, 1890) were compared with a susceptible population (control) of the same species. Standard protocols were used for biochemical estimation of enzymes. The results showed significantly higher levels of nonspecific esterases and monooxygenases in resistant spiders compared to controls. The activity of GSTs was lower in the resistant spiders. Elevated levels of nonspecific esterases and monooxygenases suggest their role in metabolic resistance in P. sumatrana. The reduced levels of total protein contents revealed its possible consumption to meet energy demands.
Keywords:resistance  spiders  metabolic enzymes  pest management
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