Presence of atrial natriuretic peptide-like material in guinea pig intestine |
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Authors: | A M Vollmar A Friedrich F Sinowatz R Schulz |
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Affiliation: | 1. Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Japan;2. AMED-CREST |
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Abstract: | Acidic extracts of guinea pig jejunum and colon contain atrial natriuretic peptide-like material (IR-ANP) detected by radioimmunoassay after purification by Sephadex G-50 gel filtration. Immunohistochemical analysis of guinea pig colon also revealed IR-ANP to be located directly beneath the lamina muscularis mucosae. High performance gel permeation chromatography (HP-GPC) and reverse phase high performance chromatography (RP-HPLC) of the IR-ANP showed correspondence to the 15 kD ANP precursor molecule (pro-ANP). No low molecular weight forms of ANP were detected. The extracted pro-ANP could be converted to alpha-ANP-like material by incubation with serum or supernatant of colonic homogenate. These data indicate the intestine to be a further site of ANP synthesis. |
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