In vitro synthesis of nerve growth factor related glioma C6 cell polypeptides. |
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Authors: | G Wiche |
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Institution: | Institute of Biochemistry, University of Vienna, Währingerstraße 17, 1090 Vienna, Austria |
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Abstract: | Rat glioma C6 cell polyribosomal preparations were tested in a heterologous system for their ability to direct the synthesis of nerve growth factor related polypeptides. Two major polypeptides of MW ~ 21,000 and ~ 43,000 respectively were found, both of which were immunoprecipitable with specific anti-mouse 2.5S nerve growth factor serum. After incubation of synthesized proteins with submaxillary gland extract the bulk of these protein species was converted into immunoprecipitable material of MW ~ 13,000, which comigrated in sodium dodecyl sulfate/polyacrylamide gel electrophoresis with mouse 2.5S nerve growth factor. |
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