Relations between auxin and cytokinin contents and in vitro rooting of tree Peony (Paeonia suffruticosa Andr.) |
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Authors: | L Bouza M Jacques B Sotta E Miginiac |
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Institution: | (1) Laboratoire de Physiologie du Développment des Plantes (C. 156), URA 1180 CNRS, Université Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France |
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Abstract: | In this work, a combined HPLC-ELISA technique was used to associate in vitro rooting capacity of tree peony micro-cuttings with contents of cytokinin and auxin; the cytokinin mainly detected corresponded to the N6-benzyladenine which had been added to the multiplication medium. Rooting capacity of explants was favoured by a preliminary accumulation of endogenous IAA only when levels of the BA absorbed from the multiplication medium had decreased. Main shoots coming from a 5-weeks subculture fulfilled these hormonal conditions and were the best microcuttings for rooting (87% rooting). Main shoots coming from shorter cycles or axillary shoots coming from a 5-weeks cycle always contained high benzyladenine levels and had a low rooting capacity (25–55% rooting). Root induction was associated with an early peak of indole-3-acetic acid followed by a 10-fold lower peak of endogenous ribofuranosyl-isopentenyladenine. Only a low and transitory accumulation of isopentenyladenine occurred during root development, and this could explain the lack of shoot development. Root development was efficient, especially in a medium containing activated charcoal, which led to an almost 3-fold decrease of IAA contents in roots.Abbreviations AC
activated charcoal
- BA
N6-benzyladenine
- ELISA
enzyme linked immunosorbent assay
- HPLC
high performance liquid chromatography
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- iP
N6-( 2-isopentenyl)adenine
- RDM
root development medium
- RIM
root induction medium
- 9RIP
9- -d-ribofuranosyl-iP
- 9RZ
9- -d ribofuranosyl-zeatin
- Z
zeatin |
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Keywords: | auxin cytokinin ELISA HPLC in vitro rooting Paeonia suffruticosa |
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