LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney |
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Authors: | R Herken M Fussek S Barth and W Götz |
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Institution: | (1) Zentrum Anatomie, Abteilung Histologie, Georg-August Universität Göttingen, Kreuzbergring 36, D-3400 Göttingen, Germany |
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Abstract: | Summary This work describes a method for the immunolocalization of laminin on 1 m-thick tissue sections using a postembedding immunofluorescence technique. Embedding of unfixed or formaldehyde-fixed mouse renal cortex in either of the acrylic resins LR-White or LR-Gold permitted reliable postembedding immunofluorescence staining for laminin. LR-White was heat-cured at 50°C whereas LR-Gold was polymerized at –25°C. A stronger immunostaining for laminin was obtained from tissue embedded in polymerized LR-Gold compared with the staining from tissue embedded in LR-White. Prerequisites for adequate postembedding immunostaining are the partial dehydration of the tissue (maximum ethanol concentration, 70%) and pepsin treatment of the tissue sections prior to performing the immunostaining reactions. |
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