Hemopexin of human and rabbit: molecular weight and extinction coefficient |
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| Authors: | V L Seery G Hathaway U M Eberhard |
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| Affiliation: | 1. The Philadelphia Hand Center, PC, Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA;1. Department of Radiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan;2. Research Park, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan;3. Department of Radiological Technology, Keio University Hospital, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan;4. Department of Radiology, Toho University Medical Center Omori Hospital, 6-11-1 Omori-nishi, Ota-ku, Tokyo, 143-8541, Japan;1. Department of Physiology and Pharmacology, University of Rome SAPIENZA, P.le A. Moro 5, 00185 Rome, Italy;2. Department of Anatomy, Histology, Forensic Medicine and Orthopedics, University of Rome SAPIENZA, via Borrelli 50, 00185 Rome, Italy;1. Department of Biomedical Engineering, The University of Texas at San Antonio, San Antonio, TX, 78249, USA;2. Department of Chemistry, The University of Texas at San Antonio, San Antonio, TX, 78249, USA;1. Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB/UNL), Oeiras, Portugal;2. Laboratory of Molecular Genetics, ITQB/UNL, Oeiras, Portugal;3. Private Pediatric Clinic, Lisbon, Portugal;4. Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, NY, USA |
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| Abstract: | The molecular weights of rabbit and human hemopexin were investigated by sedimentation equilibrium in the analytical ultracentrifuge. The apparent isopotential specific volume (φ′) of rabbit hemopexin was found to be 0.702 ± 0.003 ml/g which is very similar to the partial specific volume of 0.705 ml/g calculated from the amino acid and carbohydrate composition of both rabbit and human hemopexin. With φ′ = 0.702 ± 0.003 ml/g, the molecular weight of rabbit and human hemopexin is 57,000 ± 3,000. The extinction coefficients (E1cm1%) for rabbit hemopexin containing 1 mole of heme per mole of protein are 21.8 at 280 nm and 19.2 at 414 nm; the apoprotein has an E1cm1% of 19.7 at 280 nm. |
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