ABam HI family of tobacco highly repeated DNA: |
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Authors: | Bla?ena KoukalovÁ J Reich M Bezdêk |
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Institution: | (1) Institute of Biophysics, Czechoslovak Academy of Sciences, Královopolská 135, CS-612 65 Brno, Czechoslovakia |
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Abstract: | ABamHI family of highly repeated DNA sequences of theNicotiana tabacum nuclear genome, denoted as a HRS60-family, was recently isolated. It comprises about 2% of the tobacco nuclear genome. Monomeric
units are 182–184 bp long. Members of the HRS60-family isolated till now are closely related. DNA-DNA hybridization experiments
with DNA of the two tobacco progenitors,N. tomentosiformis andN. sylvestris, revealed that the HRS60-family was present in many copies inN. sylvestris, the amount being about 1.7 times that inN. tabacum. InN. tomentosiformis as well as in some other species of the genusNicotiana, the HRS60-family is present in a small amount. Sequences related to the HRS60-family were revealed using DNA-DNA hybridization
at low stringency. With respect to quantity, the HRS60-family could be considered as a species-specific DNA repeat which may
be a useful genetic marker in genetic manipulations withN. tabacum. |
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Keywords: | Nicotiana tabacum L Solanaceae tobacco nuclear genome |
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