1. 55 35 3299 130155 35 3299 1384;2. Department of Pathology and Parasitology, Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, MG, Brazil;3. Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, USA;4. Department of Dermatology, Harvard Medical School, Boston, USA;5. Department of Biomaterials and Oral Biology, School of Dentistry, University of S?o Paulo, S?o Paulo, SP, Brazil;6. Department of Oral Pathology, School of Dentistry, University of S?o Paulo, S?o Paulo, SP, Brazil;7. Department of Restorative Dentistry, Special Laboratory of Lasers in Dentistry (LELO), School of Dentistry, University of S?o Paulo, S?o Paulo, SP, Brazil;8. A. C. Camargo Cancer Center, National Institute of Oncogenomics and National Institute of Translational Neurosciences, S?o Paulo, SP, Brazil;9. Harvard‐MIT Division of Health Sciences and Technology, Cambridge, USA
Abstract:
Low‐level laser therapy (LLLT) has been extensively employed to improve epithelial wound healing, though the exact response of epithelium maturation and stratification after LLLT is unknown. Thus, this study aimed to assess the in vitro growth and differentiation of keratinocytes (KCs) and in vivo wound healing response when treated with LLLT. Human KCs (HaCaT cells) showed an enhanced proliferation with all the employed laser energy densities (3, 6 and 12 J/cm2, 660 nm, 100 mW), together with an increased expression of Cyclin D1. Moreover, the immunoexpression of proteins related to epithelial proliferation and maturation (p63, CK10, CK14) all indicated a faster maturation of the migrating KCs in the LLLT‐treated wounds. In that way, an improved epithelial healing was promoted by LLLT with the employed parameters; this improvement was confirmed by changes in the expression of several proteins related to epithelial proliferation and maturation.
Immunofluorescent expression of cytokeratin 10 (red) and Cyclin D1 (green) in ( A ) Control keratinocytes and ( B ) Low‐level laser irradiated cells. Blue color illustrates the nuclei of the cells (DAPI staining).
