A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue |
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| Authors: | Hugh Sparks Sean Warren Joana Guedes Nagisa Yoshida Tze Choong Charn Nadia Guerra Taranjit Tatla Christopher Dunsby Paul French |
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| Institution: | 1. Photonics Group, Physics Department, London SW7 2AZ, UK;2. Institute of Chemical Biology, SW7, UK;3. Cell and Molecular Biology, SW7, UK;4. Department of ENT‐Head &Neck Surgery, Harrow, HA1 3UJ |
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| Abstract: | Fluorescence lifetime imaging (FLIM) has previously been shown to provide contrast between normal and diseased tissue. Here we present progress towards clinical and preclinical FLIM endoscopy of tissue autofluorescence, demonstrating a flexible wide‐field endoscope that utilised a low average power blue picosecond laser diode excitation source and was able to acquire ～mm‐scale spatial maps of autofluorescence lifetimes from fresh ex vivo diseased human larynx biopsies in ～8 seconds using an average excitation power of ～0.5 mW at the specimen. To illustrate its potential for FLIM at higher acquisition rates, a higher power mode‐locked frequency doubled Ti:Sapphire laser was used to demonstrate FLIM of ex vivo mouse bowel at up to 2.5 Hz using 10 mW of average excitation power at the specimen. (© 2014 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim) |
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| Keywords: | FLIM endoscope autofluorescence lifetime fibre flexible lifetime instrument |
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