Laser Processing and Plasmonics Laboratory, Engineering Physics Department, école Polytechnique de Montréal, Montréal, Canada, Québec
Abstract:
We present a new hyperspectral reflected light microscopy system with a scanned broadband supercontinuum light source. This wide‐field and low phototoxic hyperspectral imaging system has been successful for performing spectral three‐dimensional (3D) localization and spectroscopic identification of CD44‐targeted PEGylated AuNPs in fixed cell preparations. Such spatial and spectral information is essential for the improvement of nanoplasmonic‐based imaging, disease detection and treatment in complex biological environment. The presented system can be used for real‐time 3D NP tracking as spectral sensors, thus providing new avenues in the spatio‐temporal characterization and detection of bioanalytes.
3D image of the distribution of functionalized AuNPs attached to CD44‐expressing MDA‐MB‐231 human cancer cells.
