Characterisation of rinderpest virus RNA and the action of actinomycin D on its replication |
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Authors: | K Prakash A Antony T Ramakrishnan |
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Institution: | (1) Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560 012 Bangalore |
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Abstract: | RNA extracted from purified rinderpest virus was characterised by sucrose gradient sedimentation and polyacrylamide gel electrophoresis.
The predominant virion RNA species had a sedimentation constant of 46S and its estimated molecular weight was 4.8 × 106 daltons. Consistently high amounts of UMP and AMP were detected. The melting-temperature profile of the virion RNA suggested
absence of secondary structure.
The effect of actionomycin D on the replication of rinderpest virus in Vero cells was studied by following the viral RNA synthesis
using labelled uridine as well as by infectivity titration. The viral RNA synthesis was not affected until 12 h following
infection and was inhibited thereafter between 18 and 48 h to an extent of 25% at 5 and 10 Μg levels of the drug. A 100 to
1000-fold reduction in the infectivity titres was observed in the presence of the drug. These results suggest that actinomycin
D inhibits rinderpest viral RNA replication. Sedimentation analysis of viral RNA extracted from drug-treated cultures showed
inhibition of the genome RNA of rinder-pest virus. |
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Keywords: | Rinderpest virus RNA viral RNA synthesis in infected cells infecti-vity titration sedimentation analysis |
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