A microcarrier-based cell culture process for the production of a bovine respiratory syncytial virus vaccine |
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Authors: | Moran Enda |
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Institution: | (1) MFM Laboratories Ltd., 4 Warner Dr., Springwood Industrial Estate, Braintree, Essex, CM7 2YW, UK |
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Abstract: | Veterinary viral vaccines generally comprise either attenuated or chemically inactivated viruses which have been propagated
on mammalian cell substrates or specific pathogen free (SPF) eggs. New generation vaccines include chemically inactivated
virally-infected whole cell vaccines. The NM57 cell line is a bovine nasal turbinate persistently infected (non-lytic infection)
with a strain of the respiratory syncytial virus (RSV). The potential of microcarrier technology for the cultivation in bioreactors
of this anchorage dependent cell line for RSV vaccine production has been investigated. Both Cytodex 3 and Cultispher S microcarriers
proved most suitable from a selection of microcarriers as growth substrates for this NM57 cell line. Maximum cell densities
of 4.12×105 cells ml-1and 5.52×105 cells ml-1 respectively were obtained using Cytodex 3 (3 g l-1) and and Cultispher S (1
g l-1) in 5 l bioreactor cultures. The fact that cell growth was less sensitive to agitation rate when cultured on Cultispher
S microcarriers, and that cells were efficiently harvested from this microcarrier by an enzymatic method, suggested Cultispher
S is suitable for further evaluation at larger bioreactor scales (>5 l) than that described here.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | anchorage dependent animal cell bioreactor Cultispher S Cytodex 3 microcarrier respiratory syncytial virus vaccine |
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