Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS 13C NMR and SEM |
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Authors: | Husson Eric Buchoux Sébastien Avondo Carine Cailleu Dominique Djellab Karim Gosselin Isabelle Wattraint Olivier Sarazin Catherine |
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Institution: | a Unité de Génie Enzymatique et Cellulaire, UMR 6022 CNRS - Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens Cedex, France b Plateforme Analytique, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens Cedex, France c Plateforme de Microscopie Electronique, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens Cedex, France |
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Abstract: | The supramolecular structure of four model celluloses was altered prior to their enzymatic saccharification using two ionic liquid pretreatments: one with the commonly used 1-ethyl-3-methylimidazolium acetate (Emim]+CH3COO]−) and the other with the newly developed 1-ethyl-3-methylimidazolium methylphosphonate (Emim]+MeO(H)PO2]−). The estimation of crystallinity index (CrI) by solid state 13C nuclear magnetic resonance for each untreated/pretreated celluloses was compared with the performances of their enzymatic hydrolysis. For α-cellulose, both pretreatments led to a significant decrease in CrI from 25% to 5% but had no effect on glucose yields. In contrast, The Emim]+MeO(H)PO2]− pretreatment on the long fibers of cellulose had no significant effect on the CrI although a conversion yield in glucose of 88% is obtained versus 32% without pretreatment. However, scanning electron microscopy analysis suggested a loss of fiber organization induced by both ionic liquid pretreatments leading to a larger accessibility by cellulases to the cellulose surface. |
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Keywords: | Cellulose Ionic liquid Cellulase from Trichoderma reesei Supramolecular structure Crystallinity |
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