Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures |
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Authors: | Corien Bakermans Sandra L. Tollaksen Carol S. Giometti Curtis Wilkerson James M. Tiedje Michael F. Thomashow |
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Affiliation: | (1) Center for Microbial Ecology, Michigan State University, 540 Plant and Soil Science Bldg, East Lansing, MI 48824, USA;(2) Biosciences Division, Argonne National Laboratory, Argonne, IL, USA;(3) Research Technology Support Facility, Michigan State University, East Lansing, MI, USA |
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Abstract: | It is crucial to examine the physiological processes of psychrophiles at temperatures below 4°C, particularly to facilitate extrapolation of laboratory results to in situ activity. Using two dimensional electrophoresis, we examined patterns of protein abundance during growth at 16, 4, and −4°C of the eurypsychrophile Psychrobacter cryohalolentis K5 and report the first identification of cold inducible proteins (CIPs) present during growth at subzero temperatures. Growth temperature substantially reprogrammed the proteome; the relative abundance of 303 of the 618 protein spots detected (∼31% of the proteins at each growth temperature) varied significantly with temperature. Five CIPs were detected specifically at −4°C; their identities (AtpF, EF-Ts, TolC, Pcryo_1988, and FecA) suggested specific stress on energy production, protein synthesis, and transport during growth at subzero temperatures. The need for continual relief of low-temperature stress on these cellular processes was confirmed via identification of 22 additional CIPs whose abundance increased during growth at −4°C (relative to higher temperatures). Our data suggested that iron may be limiting during growth at subzero temperatures and that a cold-adapted allele was employed at −4°C for transport of iron. In summary, these data suggest that low-temperature stresses continue to intensify as growth temperatures decrease to −4°C. |
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Keywords: | Psychrophiles Proteome Low-temperature Psychrobacter Cold acclimation |
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