Stage-specific crosstalk between light, auxin, and ethylene during low-pH-induced root hair formation in lettuce (Lactuca sativa L.) seedlings |
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Authors: | Hidenori Takahashi Yasunori Inoue |
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Institution: | (1) Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan;(2) Department of Applied Biological Sciences, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan;(3) Division of Plant Biotechnology, Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan |
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Abstract: | In the light, transfer of lettuce seedlings precultured on liquid medium at pH 6.0 to fresh medium at pH 4.0 induces root
hair formation. However, no root hairs form in the dark. Here, we investigated how light induces root hair formation. Randomization
of the transverse cortical microtubule (CMT) arrays which occurs in root epidermal cells in the light prior to root hair initiation
was not observed in the dark. However, addition of indole-3-acetic acid (IAA) or 1-aminocyclopropane-1-carboxylic acid (ACC)
induced CMT randomization and root hair formation. In these cases, CMT randomization occurred in almost the same time-dependent
manner as under light. However, root hair initiation was delayed for several hours in the dark. These results suggest that
light promotes CMT randomization and root hair initiation via auxin and ethylene signaling but light additionally influences
root hair initiation independently of these signaling mechanisms. Furthermore, addition of a microtubule-depolymerizing drug
in the dark disrupted the transverse CMT arrays and initiated root hair formation; however, root hair elongation was still
suppressed. Root hairs elongated when IAA or ACC was applied with the drug. These results suggest that light also promotes
root hair elongation via auxin and ethylene signaling. |
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Keywords: | Auxin Ethylene Lactuca sativa L Light Microtubule Root hair |
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