Kin recognition between medial Golgi enzymes in HeLa cells. |
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| Authors: | T Nilsson M H Hoe P Slusarewicz C Rabouille R Watson F Hunte G Watzele E G Berger G Warren |
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| Affiliation: | Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK. |
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| Abstract: | The medial Golgi enzymes, N-acetylglucosaminyltransferase I (NAGT I) and mannosidase II (Mann II), and the trans Golgi enzyme, beta-1,4-galactosyltransferase (GalT) were each retained in the endoplasmic reticulum (ER) by grafting on the cytoplasmic tail of the p33 invariant chain. Transient and stable expression of p33/NAGT I in HeLa cells caused relocation of endogenous Mann II to the ER and transient expression of p33/Mann II had a similar effect on endogenous NAGT I. Neither of these endogenous medial enzymes were affected by transient expression of p33/GalT. These data provide strong evidence for kin recognition between medial Golgi enzymes and suggest a role for them in the organization of the Golgi stack. |
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