A method for the determination of glucose-6-phosphatase activity in rat liver with [U-14C]glucose 6-phosphate as substrate. |
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| Authors: | S A Kitcher K Siddle J P Luzio |
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| Institution: | Department of Medical Biochemistry, Welsh National School of Medicine, Heath Park, Cardiff CF4 4XN, UK |
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| Abstract: | A method is described for measuring the activity of glucose-6-phosphatase (EC 3.1.3.9) in rat liver. U-14C]Glucose 6-phosphate, as substrate, is converted by the enzyme to 14C]glucose and inorganic phosphate. The addition of ZnSO4 and Ba(OH)2 at the end of the reaction precipitates phosphate and the unreacted 14C]glucose 6-phosphate, whereas 14C]glucose is not precipitated. After centrifugation, the amount of 14C]glucose formed is determined in a liquid scintillation counter. |
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