Long-term culture of primary rat hepatocytes with high albumin secretion using membrane-supported collagen sandwich |
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Authors: | Motoyuki Suzuki Kazuhiro Ichikawa Akiyoshi Sakoda Yasuyuki Sakai |
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Institution: | (1) Institute of Industrial Science, University of Tokyo, 7-22-1 Roppongi, 106 Minato-ku, Tokyo, Japan |
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Abstract: | Primary cultured rat hepatocytes in a membrane-supported collagen sandwich maintained their normal cell morphology and high level of albumin secretion for over 56 days. It was found that the existence of an upper layer of collagen gel is crucial for long-term culture and that the transference of cellular nutrients between the culture media and hepatocytes from both the upper and the lower sides of gel layers promotes albumin secretion. These facts suggest that the membrane-supported collagen sandwich mimics well thein vivo environment of hepatocytes. This method has great potential for the long-term culture of primary cells. |
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Keywords: | collagen extracellular matrix hepatocytes primary culture |
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