Amino acid and monoamine transport in primary astroglial cultures from defined brain regions |
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Authors: | Elisabeth Hansson Peter Eriksson Michael Nilsson |
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Institution: | (1) Institute of Neurobiology, University of Göteborg, P.O.B. 33031, S-400 33 Göteborg, Sweden |
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Abstract: | The uptake ofl-3H]glutamate,l-3H]aspartate, -3H]aminobutric acid (GABA), 3H]dopamine,dl-3H]norepinephrine and 3H]5-hydroxytryptamine (5-HT) was studied in astrocytes cultured from the cerebral cortex, striatum and brain stem of newborn rat and grown for 2 weeks in primary cultures. The astrocytes exhibited a high-affinityl-glutamate uptake withK
m values ranging from 11 to 110 M.V
max values were 4.5 in cerebral cortex, 39.1 in striatum, and 0.4 in brain stem, nmol per mg cell protein per min. There was a less prominent high-affinity uptake ofl-aspartate withK
m values from 88 to 187 M.V
max values were 7.4 in cerebral cortex, 37.1 in striatum, and 3.1 in brain stem, nmol per mg cell protein per min. The high-affinity GABA uptake exhibitedK
m values ranging from 5 to 17 M andV
max values were 0.01 for cerebral cortex, 0.04 for striatum, and 0.1 for brain stem, nmol per mg cell protein per min. No high-affinity, high-capacity uptake was found for the monoamines. The results demonstrate a heterogeneity among the astroglial cells cultivated from the different brain regions concerning the uptake capacity of amino acid neurotransmitters. Furthermore, amino acid transmitters and monoamines are taken up by the cells in different ways. |
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Keywords: | |
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