| 应用ARDRA技术研究Frankia菌多样性 |
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| 引用本文： | 刘忠,吴少慧,周斌,张惠文,张忠泽. 应用ARDRA技术研究Frankia菌多样性[J]. 微生物学杂志, 2001, 21(1): 20-23 |
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| 作者姓名： | 刘忠 吴少慧 周斌 张惠文 张忠泽 |
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| 作者单位： | 中国科学院沈阳应用生态研究所, |
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| 基金项目： | 国家自然科学基金资助项目(39870038);中科院系统与进化特别项目资助 |
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| 摘 要： | 应用原核生物16SrDNA特异性引物rD1和fD1,对分自4个分类接种群的12株纯培养Frankia菌总DNA进行扩增,得到1条长约1500bp的扩增产物.选用2种内切酶HinfI,MspI对扩增产物进行酶切,得到稳定的酶切图谱.对图谱的分析结果表明,Frankia菌间存在极其丰富的遗传多样性.
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| 关 键 词： | Frankia 多样性 ARDRA |
| 文章编号： | 1005-7021(2001)01-0020-04 |
| 修稿时间： | 2000-11-05 |
Study on Genetic Diversity of Frankia by ARDRA |
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| Liu Zhong et al.. Study on Genetic Diversity of Frankia by ARDRA[J]. Journal of Microbiology, 2001, 21(1): 20-23 |
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| Authors: | Liu Zhong et al. |
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| Abstract: | S ribosomal DNAs (rDNAs) from 12 Frankia strains belonging to 4 taxonomic inoculation groups were amplified by PCR with primer rD1 and fD1. The fragment of 16s rDNA with about 1 500 bp was obtained. The amplification products were digested by Hinff and MspI respectively. It showed that there are great genetic diversity among Frankia strains by comparing the patterns from the digestion. |
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| Keywords: | Frankia diversity ARDRA. |
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