Identification and cloning of a umu locus in Streptomyces coelicolor A3(2) |
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| Authors: | F Misuraca D Rampolla S Grimaudo |
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| Institution: | Dipartimento di Biologia Cellulare e dello Sviluppo A. Monroy, University of Palermo, Italy. |
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| Abstract: | The umuDC operon of Escherichia coli is required for efficient mutagenesis by UV and many other DNA-damaging agents. E. coli umu mutants are defective in mutagenesis and slightly more sensitive to DNA-damaging agents. The existence of a umuDC analogue in Streptomyces coelicolor was suggested by data of our previous works. We cloned from Streptomyces coelicolor a fragment of DNA homologous to the E. coli umuDC region that is able to complement the E coli umuC122::Tn5 mutation. Therefore our data suggest that S. coelicolor contains a functional umu-like operon. |
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