| 一种用于研究骨骼肌缺血/再灌注损伤的细胞模型 |
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| 作者姓名： | Li HJ Zhang GL Zhang LY Dong SY Men XL |
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| 作者单位： | 华北煤炭医学院基础部病理生理教研室,河北,唐山,063000 |
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| 摘 要： | 目的:复制L-6TG大鼠肌母细胞缺血/再灌注损伤的细胞模型.方法:将培养的L-6TG大鼠肌母细胞随机分为2组:①正常对照组(C组),②缺血/再灌注组(I/R组),观测了培养上清中乳酸脱氢酶(LDH)、细胞内超氧化物歧化酶(SOD)、黄嘌呤氧化酶(XOD)、Ca2 含量的变化;采用MTT法检测线粒体的功能;在光镜下观察细胞的形态学改变.结果:与对照组相比,L-6TG大鼠肌母细胞IR 4h后培养上清中LDH、细胞内XOD、Ca2 含量明显增加,细胞内SOD及线粒体呼吸功能明显降低,细胞严重受损,明显圆缩,并有脱落现象.结论:应用模拟缺血液和再灌液可成功复制L-6TG大鼠肌母细胞缺血/再灌注损伤的细胞模型.
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| 关 键 词： | 缺血/再灌注 细胞培养 |
| 文章编号： | 1000-6834（2005）03-0356-03 |
| 收稿时间： | 2004-06-21 |
| 修稿时间： | 2004-06-212005-03-22 |
A cell model of ischemia/reperfusion injury on skeletal muscle |
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| Li HJ,Zhang GL,Zhang LY,Dong SY,Men XL.A cell model of ischemia/reperfusion injury on skeletal muscle[J].Chinese Journal of Applied Physiology,2005,21(3):356-358,i0005. |
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| Authors: | Li Hong-Jie Zhang Guang-Ling Zhang Lian-Yuan Dong Shu-Yun Men Xiu-Li |
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| Institution: | Department of Pathophysiology of North China Coal Medical College, Tangshan 063000, China. |
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| Abstract: | Aim: To establish a model of ischemia/reperfusion injury on L-6TG cell. Methods: Cultured L-6TG cells were divided into 2 groups: control group(C),ischemia/reperfusion group(I/R),LDH in culture fluid,SOD, XOD, free calcium in L-6TG cell and mitochondria respiration were evaluated in each group, the micromorphologic changes were observed with microscope. Results: Compared with control group, after L-6TG cell suffered ischemia 4 hours and reperfusion 4 hours, LDH in culture fluid, XOD, free calcium in L-6TG cell all increased significantly, while SOD in L-6TG cell and mitochondrial respiration decreased, structural damage to L-6TG cell was severe. Conclusion:WTBZ] Using mimicking ischemic solution and mimicking reperfusion solution can successfully establish a model of ischemia/reperfusion injury on L-6TG cell. |
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| Keywords: | ischemia/reperfusion injury cell culture |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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