在基因家族背景下对四种植物中DGAT2的鉴定和序列分析

二酰甘油酰基转移酶2(Diacylglycerol O-acyltransferase 2,DGAT2)是植物中三羧酸甘油酯(TAG)合成途径的限速酶,其编码基因属于酰基转移酶基因超家族。本研究依托植物全基因组数据库Phytozome,通过BLAST搜索获得了蓖麻(Ricinus communis L.)、拟南芥(Arabidopsis thaliana Heynh.)、毛果杨(Populus tricho-carpa Torr.A.Gray.)和木薯(Manihot esculenta Crantz.)4种双子叶植物酰基转移酶基因超家族所编码的73条多肽序列,并从中鉴定出5条DGAT2序列。理化性质和跨膜结构域分析表明,5条DGAT2序列均为疏水性跨膜蛋白,其中木薯DGAT2为一次跨膜蛋白且在叶绿体膜中大量分布,这与其他植物的DGAT2序列存在差异;木薯DGAT2蛋白在进化过程中发生了功能分化且可能与木薯的抗逆作用有关。

Identification and Sequence Analysis of DGAT2s in Four Plants under the Scenario of Gene Family

DGAT2, a rate-limiting enzyme of the TAG biosynthesis pathway, was coded by the acyltransferase supergene family. Genome-wide analysis revealed 73 acyltransferase supergene family members and five DGAT2s in the genomes of Ricinus communis L., Arabidopsis thaliana Heynh., Populus trichocarpa Torr. & A. Gray. and Manihot esculenta Crantz. based on the whole genome database Phytozome. Analysis of the physical and chemical properties and transmembrane domains indicated that DGAT2s were hydrophobic transmembrane proteins, and DGAT2s of M.esculenta differed from the others due to their single transmembrane domain and wide chloroplast membrane distribution. We concluded that functional differentiation of M.esculenta DGAT2s may exist and DGAT2s may be involved in the anti-stress pathways in M.esculenta.