Molecular characterization of Ascaris suum DNA and of chromatin diminution |
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| Authors: | Paul Goldstein Neil A. Straus |
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| Affiliation: | 1. Department of Biology, York University, Downsview, Ont., Canada;2. Department of Botany, University of Toronto, Toronto, Ontario, Canada |
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| Abstract: | A technique for the extraction of pure somatic (post-diminution) and germ line (pre-diminution) DNA from the parasitic nematode Ascaris is described. Uncontaminated post- and pre-diminution DNAs were sheared and reassociated to different C0t values. Computer analysis of the complete reassociation kinetics determined that 33% of the germ line genome is eliminated during the process of chromatin diminution. The eliminated DNA is comprised of repetitive and unique sequences in an approx. 1:1 ratio. |
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