Efficiency of Neutral Red,Evans Blue and MTT to assess viability of the freshwater microalgae Desmodesmus communis and Pediastrum boryanum |
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Authors: | Danieli Saul Da Luz Daniele Gomes Da Silva Marta Marques Souza Danilo Giroldo Camila De Martinez Gaspar Martins |
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Affiliation: | 1. Programa de Pós‐Gradua??o em Biologia de Ambientes Aquáticos Continentais;2. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande – FURG, Rio Grande, RS, Brazil |
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Abstract: | This study assessed the efficacy of three cell viability assays – Methyl‐thiazolyl‐tetrazolium (MTT), Evans Blue, and Neutral Red – for two freshwater microalgae species, Desmodesmus communis and Pediastrum boryanum (Chlorophyceae), in order to find suitable techniques to detect the levels of pollution in water ecosystems. Following exposure to a glyphosate‐based herbicide, our results showed that Evans Blue did not adequately measure cell viability in either species, while MTT and Neutral Red were able to detect decreased cellular viability for both algae in response to herbicide exposure. Overall, however, Neutral Red proved to be more sensitive than MTT for these algae. |
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Keywords: | Algae cell viability Chlorophyceae Desmodesmus Evans Blue MTT Neutral Red Pediastrum |
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