Measuring gene expression by quantitative proteome analysis |
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Authors: | Gygi S P Rist B Aebersold R |
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Institution: | Department of Molecular Biotechnology, University of Washington, Seattle 98195-7730, USA. gygi@u.washington.edu |
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Abstract: | Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the precise quantification and identification of proteins in complex mixtures, that is, quantitative proteomics. |
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