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Measuring gene expression by quantitative proteome analysis
Authors:Gygi S P  Rist B  Aebersold R
Institution:Department of Molecular Biotechnology, University of Washington, Seattle 98195-7730, USA. gygi@u.washington.edu
Abstract:Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the precise quantification and identification of proteins in complex mixtures, that is, quantitative proteomics.
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