Ultraviolet scanning densitometry for detection, quantitation, and preparative elution of protein bands from unstained gels |
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| Authors: | P Bartolini R Arkaten M T Ribela |
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| Institution: | Department of Application of Nuclear Techniques in Biological Sciences, Cidade Universitaria, S?o Paulo, Brazil. |
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| Abstract: | Ultraviolet scanning of gel rods was used to identify and quantify protein bands in a nondestructive manner with good precision and sensitivity. This same technique, applied on a preparative scale, allowed quantitative protein elution, by reversed electrophoresis, from gel slices completely sealed in a dialysis bag. Protein recovery approached the theoretical yield (93.5 +/- 5%), with practically no interfering substances, and the entire preparative process (first electrophoresis, densitometric scanning, and reversed electrophoresis) could be performed in approximately 6 h. Its application to human growth hormone has shown no alteration in the biological activity of this protein. |
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