Development of simple sequence repeat (SSR) markers and construction of an SSR-based linkage map in Italian ryegrass (Lolium multiflorum Lam.) |
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| Authors: | Mariko Hirata Hongwei Cai Maiko Inoue Nana Yuyama Yuichi Miura Toshinori Komatsu Tadashi Takamizo Masahiro Fujimori |
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| Institution: | (1) Forage Crop Research Institute, Japan Grassland Agriculture and Forage Seed Association, 388-5 Higashiakada, Nasushiobara, Tochigi 329-2742, Japan;(2) National Institute of Livestock and Grassland Science, 768 Senbonmatsu, Nishinasuno, Tochigi 329-2747, Japan;(3) Yamanashi Prefectural Dairy Experiment Station, 621-2 Nagasaka-Kamijo, Nagasaka, Hokuto, Yamanashi 408-0021, Japan |
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| Abstract: | In order to develop simple sequence repeat (SSR) markers in Italian ryegrass, we constructed a genomic library enriched for (CA)n-containing SSR repeats. A total of 1,544 clones were sequenced, of which 1,044 (67.6%) contained SSR motifs, and 395 unique clones were chosen for primer design. Three hundred and fifty-seven of these clones amplified products of the expected size in both parents of a two-way pseudo-testcross F1 mapping population, and 260 primer pairs detected genetic polymorphism in the F1 population. Genetic loci detected by a total of 218 primer pairs were assigned to locations on seven linkage groups, representing the seven chromosomes of the haploid Italian ryegrass karyotype. The SSR markers covered 887.8 cM of the female map and 795.8 cM of the male map. The average distance between two flanking SSR markers was 3.2 cM. The SSR markers developed in this study will be useful in cultivar discrimination, linkage analysis, and marker-assisted selection of Italian ryegrass and closely related species.Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users. |
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