Moderately Elevated Temperature Eliminates Resistance of Rice Plants with Enhanced Expression of Glutathione Reductase to Intensive Photooxidative Stress |
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Authors: | Kouřil R Lazár D Lee H Jo J Nauš J |
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Institution: | (1) Laboratory of Biophysics, Faculty of Science, Palacký University, t , Svobody 26, 771 46 Olomouc, Czech Republic;(2) Department of Animal Science, Kyungpook National University, Daegu, 702701, South Korea |
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Abstract: | We developed transgenic rice plants (Oryza sativa L. cv. Daeribbyeo) overproducing cytosolic glutathione reductase (GR) using a GR gene from Brassica campestris and studied their response to photo-oxidative stress in the presence of methyl viologen (MV, 10 and 50 M concentrations) under room (25 °C) and moderately elevated (35 °C) temperature by analysis of chlorophyll (Chl) a fluorescence parameters (FV/FM, qN, and qP) and of Chl content. Elevated temperature enhanced and accelerated the photo-oxidative damage to photosynthetic apparatus expressed mainly by a fast decrease of qN. Higher temperature supported the protective reaction in transformed rice plants for lower MV concentration (10 M) and eliminated the enhanced tolerance of photosystem 2 photochemistry to photooxidative stress for higher (50 M) MV concentration. Different mechanisms and temperature dependence of oxidative and protective reactions explain the results. |
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Keywords: | active oxygen species chlorophyll fluorescence Oryza sativa photochemical and non-photochemical fluorescence quenching transgenic plants |
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