GAWK, a novel human pituitary polypeptide: isolation, immunocytochemical localization and complete amino acid sequence |
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Authors: | S Benjannet R Leduc C Lazure N G Seidah M Marcinkiewicz M Chrétien |
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Affiliation: | 1. Biosensor Sciences and Technologies Research Center, Ardabil University of Medical Sciences, Ardabil, Iran;2. Department of Medicinal Chemistry, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran;3. Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran;4. Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, Iran |
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Abstract: | During the course of reverse-phase high pressure liquid chromatography (RP-HPLC) purification of a postulated big ACTH (1) from human pituitary gland extracts, a highly purified peptide bearing no resemblance to any known polypeptide was isolated. The complete sequence of this 74 amino acid polypeptide, called GAWK, has been determined. Search on a computer data bank on the possible homology to any known protein or fragment, using a mutation data matrix, failed to reveal any homology greater than 30%. An antibody produced against a synthetic fragment allowed us to detect several immunoreactive forms. The antisera also enabled us to localize the polypeptide, by immunocytochemistry, in the anterior lobe of the pituitary gland. |
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