Synthesis of carrier-free β-32P-nucleosides-triphosphate in almost quantitative yields |
| |
| Authors: | Gabriel Kaufmann Mordechai Choder Yoram Groner |
| |
| Institution: | 1. Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel;2. Department of Virology, The Weizmann Institute of Science, Rehovot, Israel |
| |
| Abstract: | The synthesis of carrier-free β-32P-ribonucleosides triphosphate in almost quantitative yields is described. The procedures are based on a polynucleotide phosphorylase-catalyzed phosphorolysis of either poly- or oligoribonucleotides with carrier-free 32P-inorganic phosphate, coupled with a pyruvate kinase-catalyzed phosphorylation of the resulting β-32P-nucleoside diphosphate. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
