N-(β-iodoethy)trifluoroacetamide: A new reagent for the aminoethylation of thiol groups in proteins |
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| Authors: | Warren E. Schwartz Paul K. Smith Garfield P. Royer |
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| Affiliation: | 1. Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA;1. Pierce Chemical Company, Box 117, Rockford, Illinois 61105 USA |
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| Abstract: | β-Iodoethyltrifluoroacetamide was prepared and evaluated for use in the aminoethylation of sulfhydryl groups in proteins. Lysozyme, ribonuclease A, chymotrypsinogen A, and soybean trypsin inhibitor were reduced using dithiothreitol in 6 m guanidine hydrochloride. The reduced proteins were then treated with the reagent. Conversion of cysteinyl to aminoethylcysteinyl residues averaged 97%; no destruction of nontarget residues was observed. |
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| Keywords: | To whom correspondence should be addressed. |
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