Convenient rapid determination of picomole amounts of oxaloacetate and aspartate |
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| Authors: | R. Parvin M.N.V. Caramancion S.V. Pande |
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| Affiliation: | Laboratory of Intermediary Metabolism, Clinical Research Institute of Montreal, Montreal H2W 1R7, Quebec, Canada |
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| Abstract: | The assay of oxaloacetate based on the citrate synthase catalyzed conversion of labeled acetyl-CoA to citrate has been greatly simplified by the development of a charcoal separation method for the selective adsorption of acetyl-CoA. An application of this procedure for the determination of oxaloacetate in rat livers is described. By coupling to glutamate oxaloacetate transaminase, the procedure enables determination of aspartate. It allows also a sensitive assay of glutamate oxaloacetate transaminase activity. |
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