Alcohol dehydrogenase-coupled spectrophotometric assay of epoxide hydratase activity |
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Authors: | FPeter Guengerich Patricia S Mason |
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Institution: | 1. Department of Biochemistry, Vanderhilt University School of Medicine, Nashville, Tennessee 37232 USA;2. Center in Environmental Toxicology, Vanderhilt University School of Medicine, Nashville, Tennessee 37232 USA |
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Abstract: | A coupled assay was devised for the assay of liver microsomal epoxide hydratase using the ability of alcohol dehydrogenase to transfer electrons from diols to NAD+: epoxide hydratase activity was continuously monitored at 340 nm. Rates of hydrolysis of octene-1,2-oxide and styrene-7,8-oxide measured utilizing this assay were similar to those determined using gas-liquid chromatography and radiometric thin-layer chromatography, respectively. The assay was used to examine the ability of rat liver microsomes and highly purified rat liver microsomal epoxide hydratase fractions to hydrolyze 15 other epoxides. |
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