A stopped-flow laser turbidimeter for studying changes in the shape of cells stimulated by external agents |
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Authors: | David A. Deranleau Christoph Rothen Marcus Streit Daniel Dubler Ernst F. Lüscher |
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Affiliation: | University of Berne, Theodor Kocher Institute, Bern, Switzerland |
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Abstract: | A laser turbidimeter suitable for following rapid morphological changes which may accompany the stimulation of cells suspended in an adequate medium is described. Stopped-flow mixing is used to initiate the reaction and the changes in light transmission are followed with a fast recording technique. The apparatus has been used to examine the ADP-induced shape change reaction of human blood platelets. |
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